Journal: International Journal of Nanomedicine
Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
doi: 10.2147/IJN.S516605
Figure Lengend Snippet: TFF2 is specifically expressed in sEVs derived from GJ. ( A ) The particle size distribution of sEVs derived from GJ in mice and human. ( B ) The morphological characteristics of gsEVs derived from mice and humans were observed by TEM. ( C ) The sEVs markers, non-sEVs marker, and TFF2 were detected in GJ, ultracentrifugation supernatant, and gsEVs derived from mice. ( D ) The sEVs markers, non-sEVs marker, and TFF2 were detected in GJ, ultracentrifugation supernatant, and gsEVs derived from human. ( E and F ) The expression levels of membrane markers of sEVs (CD9, CD63 and CD81) and TFF2 was detected by bead-bound based flow cytometry in human gsEVs. ( G )The expression levels of sEV markers, including CD63, Syntenin-1, and TFF2, were analyzed in human gsEVs using immunogold labeling and TEM.
Article Snippet: In the UC and PEG-UC methods, 20 μg of protein was loaded; in the UC-SEC and UF-SEC methods, 20 μL of each fraction was loaded. ( E ) The membrane markers of sEVs (CD9, CD63 and CD81) were detected by NanoFCM.
Techniques: Derivative Assay, Marker, Expressing, Membrane, Flow Cytometry, Labeling